Our Guide to Reconstitution
Reconstitution is the process of dissolving lyophilised (freeze-dried) peptide powder into a liquid solution using bacteriostatic water. The amount of water you add determines the concentration, which affects how much liquid you draw per dose.
Below you'll find peptide-specific reconstitution options with at least two BAC water volumes per product, so you can choose the concentration that best fits your research protocol.
Clean Both Vials
Swab the rubber stopper of both the peptide vial and the BAC water vial with an alcohol swab. Allow to air dry for 10 seconds.
Draw BAC Water
Using a clean insulin syringe, draw your chosen volume of BAC water. Pull the plunger slowly and steadily to minimise air bubbles.
Add Against the Vial Wall
Insert the needle into the peptide vial. Angle the needle so the water runs down the inside wall of the vial — never squirt directly onto the powder. Release slowly.
Dissolve Gently
Never shake. Gently swirl or roll the vial between your palms. Let it sit for 2–3 minutes if needed. The solution should be completely clear.
Refrigerate Immediately
Store at 2–8°C. Label the vial with the date and concentration. Use within 28–30 days. Never freeze a reconstituted peptide.
Jump to a peptide:
Retatrutide
Triple AgonistRetatrutide is dosed in milligrams, typically ranging from 1mg (starting) up to 12mg (maintenance). The BAC water volume you choose depends on which dose range you're working with — lower concentrations give easier measurements at lower doses, higher concentrations reduce injection volume at higher doses.
10mg Vial
20mg Vial
30mg Vial
Storage: Lyophilised: -20°C long-term, 2–8°C short-term. Reconstituted: 2–8°C, use within 28 days. Never freeze after reconstitution.
Tirzepatide
Dual AgonistTirzepatide is dosed weekly, typically starting at 2.5mg and titrating up to 15mg. Clinical protocols use dose escalation every 4 weeks.
Storage: Lyophilised: -20°C long-term. Reconstituted: 2–8°C, use within 28 days. Molecular weight: ~4,813 Da.
Semaglutide
GLP-1 AgonistSemaglutide is dosed weekly, typically starting at 0.25mg and titrating up to 2.4mg over several months. The low starting dose makes concentration choice critical for accurate measurement.
Note: At 5mg/ml concentration, a 0.25mg starting dose is only 5 units — which is difficult to measure accurately on a standard 100-unit syringe. The 2ml option (2.5mg/ml) is strongly recommended for researchers starting at low doses.
Storage: Lyophilised: -20°C long-term. Reconstituted: 2–8°C, use within 28 days. Molecular weight: ~4,113 Da.
Melanotan II
Melanocortin AgonistMelanotan II is typically dosed in micrograms (mcg), with common research doses ranging from 250mcg to 1mg. Lower starting doses are common for initial tolerance assessment.
Loading vs maintenance: Research protocols typically use a higher frequency (daily) during an initial loading phase, then reduce to 1–2 times per week for maintenance. The 2ml option gives better measurement precision for the lower maintenance doses.
Storage: Lyophilised: -20°C. Reconstituted: 2–8°C, use within 28 days. Protect from UV light. MW: 1,024.18 g/mol.
Ipamorelin
GH SecretagogueIpamorelin is typically dosed at 200–300mcg per administration, often 2–3 times daily on an empty stomach. The pentapeptide structure makes it very stable once reconstituted.
Timing matters: Ipamorelin is most effective on an empty stomach — typically 30 minutes before eating or 2+ hours after a meal. GH secretagogues are sensitive to blood glucose levels.
Storage: Lyophilised: -20°C. Reconstituted: 2–8°C, use within 28 days. MW: 711.85 g/mol.
Wolverine Blend
BPC-157 + TB-500The Wolverine Blend contains 5mg BPC-157 + 5mg TB-500 (10mg total) in a single vial. BPC-157 is typically dosed at 250–500mcg/day while TB-500 is typically dosed at 2–5mg twice weekly during loading and 1–2mg weekly for maintenance. Because they're blended, each draw contains both peptides in a 1:1 ratio.
Blend note: Since BPC-157 and TB-500 are pre-mixed at a 1:1 ratio, each dose delivers equal amounts of both peptides. If your protocol requires different ratios, purchase them as separate vials.
Storage: Lyophilised: -20°C. Reconstituted: 2–8°C, use within 28 days.
Glow Blend
GHK-Cu + BPC-157 + TB-500The Glow Blend contains 50mg GHK-Cu, 10mg BPC-157, and 10mg TB-500 (70mg total). GHK-Cu dominates the blend as it requires higher doses for skin and collagen research protocols.
Appearance: GHK-Cu solution will appear blue/blue-green after reconstitution — this is completely normal and indicates active copper binding. A clear, colourless solution suggests the copper complex may be compromised.
Storage: Lyophilised: -20°C, protect from light. Reconstituted: 2–8°C, use within 28 days.
GHK-Cu (Copper Peptide)
RegenerativeStandalone GHK-Cu (50mg) is dosed in milligrams, with common research doses ranging from 1–3mg per administration, typically once daily. Higher doses are used in some dermatological research protocols.
Appearance: Reconstituted GHK-Cu will appear blue — this is the natural colour of the copper-peptide complex and indicates proper binding. MW: 403.92 g/mol.
Storage: Lyophilised: -20°C, protect from light. Reconstituted: 2–8°C, use within 28 days.
Golden Rules of Reconstitution
1. Always add BAC water against the vial wall — never directly onto the powder.
2. Never shake — gently swirl or roll between your palms.
3. Refrigerate immediately after reconstitution (2–8°C).
4. Use within 28–30 days of reconstitution.
5. Never freeze a reconstituted peptide.
6. Label every vial with the date, peptide name, and concentration.
All products sold by Tidal Peptides are intended strictly for laboratory and research use only. Not for human consumption. The information on this page is for educational purposes only and does not constitute medical advice. Always follow your specific research protocol requirements.